荧光钙探针 Fura 2-AM CAS#: 108964-32-5

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荧光钙探针 Fura 2-AM

生物活性体外研究 用途与合成方法 MSDS 荧光钙探针 Fura 2-AM价格(试剂级) 上下游产品信息

中文名称	荧光钙探针 Fura 2-AM
中文同义词	钙离子荧光探针;钙荧光探针FURA-2, AM;钙离子荧光探针 Fura 2-AM;荧光钙探针 Fura 2-AM;AM荧光探针;FURA-2/AM荧光探针;FURA 2-AM 1MM/L DMSO SOLUTION;FURA-2,AM,荧光钙离子指示剂
英文名称	Fura 2-AM
英文同义词	FURA 2/AM;FURA-2 PENTAKIS(ACETOXYMETHYL) ESTER;FURA 2/AM in Solution - CAS 108964-32-5 - Calbiochem;FURA-2/AM, FOR FLUORESCENCE;FURA 2-AM SPECIAL PACKAGING;FURA 2/AM in Solution;fura 2-am yellow powder;Fura?-AM
CAS号	108964-32-5
分子式	C44H47N3O24
分子量	1001.85
EINECS号
相关类别	荧光探针，标记，颗粒和染色;分析试剂-显色剂;生化试剂;荧光染料;Benzofuran
Mol文件	108964-32-5.mol
结构式

荧光钙探针 Fura 2-AM 性质

荧光钙探针 Fura 2-AM 用途与合成方法

生物活性

Fura-2 AM 是一种高亲和力，细胞内，紫外光激发和 UV 比率荧光 Ca2+ 指示剂。

体外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).  
 Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.  
 1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.  
 2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.  
 3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.  
 4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.  
 5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.  
 6. Mount the coverslip on the imaging chamber.

安全信息

MSDS信息

荧光钙探针 Fura 2-AM 价格(试剂级)

荧光钙探针 Fura 2-AM 上下游产品信息