钙黄绿素乙酰甲酯 CAS#: 148504-34-1

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钙黄绿素乙酰甲酯

生物活性体外研究体内研究 用途与合成方法 MSDS 钙黄绿素乙酰甲酯价格(试剂级) 上下游产品信息

中文名称	钙黄绿素乙酰甲酯
中文同义词	钙黄绿素,AM;黄绿素-AM;细胞染色剂黄绿素-AM;钙黄绿素, AM *超级纯*;钙黄绿素乙酰甲酯;Calcein，AM,≥96.0% (HPLC)用于荧光分析 （1mg  1119.00）;钙黄绿素乙酰甲酯 CALCEIN, AM,≥96.0% (HPLC);CELLSTAIN CALCEIN AM SOLUTION(1MM/L DMSO) (3',6'-DI(O-ACETYL)-4',5'-BIS[N,N-BIS(CARBOXYMETHY
英文名称	CALCEIN-AM
英文同义词	3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution;Calcein  AM  solution;CALCEIN, AM;CAL-AM;4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester;Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester;Calcein AcetoxyMethyl Ester;N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester;NSC 689290
CAS号	148504-34-1
分子式	C46H46N2O23
分子量	994.86
EINECS号	200-664-3
相关类别	荧光探针，标记，颗粒和染色;荧光染料;细胞生物学试剂;生化试剂;Amines;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Xanthene;CCalcium  Signaling  Research  Tools;Calcium  Probes;Fluorescent;Stains  and  Dyes;Stains&Dyes,  A  to
Mol文件	148504-34-1.mol
结构式

钙黄绿素乙酰甲酯 性质

钙黄绿素乙酰甲酯 用途与合成方法

生物活性

Calcein-AM是用于测定细胞活力的可以渗透细胞的荧光染料。

体外研究

The calcein-AM dye used to stain the living cells is shown to have a low spontaneousleakage rate less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM has a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this microtest. Quantitation of killing and kinetic analysis is readily performed with the test system. Calcein-AM is pH independent, better retained and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with intact plasma membrane. From dying or damaged cells with compromised membrane integrity or from cells expressing multidrug resistance protein (MRP), unhydrolyzed substrates and their fluorescent products are rapidly extruded from cells. The calcein-AM assay has been used to assess the cell viability, cytotoxicity and tp quantitate apoptosis.

体内研究

Calcein-AM is found to be suitable for     in vivo    studies, because it has no deleterious effects on cell function and is, indeed, a marker of cell viability.

安全信息

MSDS信息

钙黄绿素乙酰甲酯 价格(试剂级)

钙黄绿素乙酰甲酯 上下游产品信息