荧光素 CAS#: 2321-07-5

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荧光素

生物活性体外研究 用途与合成方法 MSDS 荧光素价格(试剂级) 上下游产品信息 专题

中文名称	荧光素
中文同义词	荧光橙红;荧光素, 指示剂;黄 7 [CI 45350:1];荧光素,水溶;荧光酯;萤光橙黄;萤光红;萤光黄
英文名称	Fluorescein
英文同义词	FLUORESCEIN, FREE ACIDFLUORESCEIN, FREE ACIDFLUORESCEIN, FREE ACID;Diresorcinolphthalein;Tetraoxyphthalophenone anhydride;SOLVENT YELLOW 94;S NO 880;RESORCINOL PHTHALEIN;FLUORESCEIN, ALCOHOL SOLUBLE;FLUORESCEIN
CAS号	2321-07-5
分子式	C20H12O5
分子量	332.31
EINECS号	219-031-8
相关类别	滴定;分析试剂;标准品;色素类;生化试剂;生物染色剂;小分子抑制剂;基因组学和分子诊断;化合物;生化试剂-色素类;化学试剂;化工原料;E-FFluorescent  Probes,  Labels,  Particles  and  Stains;Fluorescein  and  Derivatives;Fluorescent  Labels;Stains  and  Dyes;Stains&Dyes,  A  to;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;FUNDUSCEIN;Fluorescent;Xanthene;大化工;化工材料;有机试剂;通用生化试剂-生物染色剂与化学指示剂;染料;试剂盒;日化品原料;有机化工原料
Mol文件	2321-07-5.mol
结构式

荧光素 性质

荧光素 用途与合成方法

生物活性

Fluorescein (Resorcinol phthalein) 是一种合成的有机光敏染料化合物。Fluorescein 是荧光示踪剂，可以应用于医学和生物学以及肿瘤感染的组织。

体外研究

Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays.  
 The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.

化学性质 

荧光素外观呈橙黄或浅橙红色结晶粉末。熔点314-316℃（分解）。溶于热乙醇、冰醋酸、碱溶液，呈绿色萤光，不溶于水、苯、氯仿和乙醚。

用途 

荧光素是汞溴红的中间体，可用作萤光光度分析试剂，测定氯、溴、碘、硫离子；氧化-还原指示试剂；生物染色剂。

用途 

荧光光度分析硫离子，试验溴，滴定氯、溴和碘。吸附指示剂，氧化还原指示剂。

用途 

吸附指示剂；氧化还原指示剂；荧光光度分析硫离子；滴定氯、溴和碘

生产方法 

荧光素可用邻苯二甲酸酐与间苯二酚在硫酸参与下反应制得。

安全信息

MSDS信息

荧光素 价格(试剂级)

荧光素 上下游产品信息

上游原料

苯酐间苯二酚间苯二酚苯酐

下游产品

赤藓红B异硫氰酸荧光素3',6'-二氯荧光素3',6'-二甲氧基荧烷曙红(醇溶)3',6'-BIS{[4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)OXY]-3H-[SPIRO(ISOBENZOFURAN-1,9,-XANTHEN]-3-ONE}FBBBE荧光素二丁酸酯溶剂红 73 [CI 45425:1]