2-硝基苯-beta-D-半乳糖苷 CAS#: 369-07-3

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2-硝基苯-beta-D-半乳糖苷

生物活性体外研究 用途与合成方法 MSDS 2-硝基苯-beta-D-半乳糖苷价格(试剂级) 上下游产品信息 专题

中文名称	2-硝基苯-beta-D-半乳糖苷
中文同义词	2-硝基苯基-~-D-半乳糖苷, 98+%;邻硝基苯-Α-D-吡喃葡萄糖苷/2-硝基苯-Α-D-吡喃葡萄糖苷/ONPG;2-硝基苯基-Β-D-吡喃半乳糖苷(动物源);邻硝基苯-BETA-D-半乳糖苷;2-硝基苯基-Β-D-吡喃半乳糖苷(植物源);2-硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Β-D-半乳糖苷(ONPG)
英文名称	2-Nitrophenyl-beta-D-galactopyranoside
英文同义词	2-Nitrophenyl hexopyranoside;2-Nitrophenyl-beta-delta-galactopyranoside;2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol;o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem;beta-D-Galactopyranoside, 2-nitrophenyl;beta-d-galactopyranoside,2-nitrophenyl;Galactopyranoside, o-nitrophenyl, beta-D-;o-Nitrophenyl beta-galactoside
CAS号	369-07-3
分子式	C12H15NO8
分子量	301.25
EINECS号	206-716-1
相关类别	化学试剂;分子生物试剂-多肽与蛋白;碳水化合物;专业试剂;酶类;中间体;氨基酸;核苷;酶底物;糖类化合物;糖类;检测标志试剂;糖类（碳水化合物）;Substrates;Biochemistry;Galactose;Glycosides;Enzyme  substrates;Carbohydrates & Derivatives;生化试剂;糖苷系列;多肽与蛋白;酶活性检测;酶及活性检测;试剂;优势产品;生化试剂-酶类;即用型溶液;其他原料;精细化工原料;化学原料;通用生化试剂-酶与辅酶;还原剂;化工中间体;其它;化学试剂;Sugars;substrate
Mol文件	369-07-3.mol
结构式

2-硝基苯-beta-D-半乳糖苷 性质

2-硝基苯-beta-D-半乳糖苷 用途与合成方法

生物活性

ONPG 是一种比色和分光光度计底物，用于检测 β-半乳糖苷酶活性。

体外研究

The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (K     _m    ), turnover numbers (k     _cat    ), and catalytic efficiencies (k     _cat    /K     _m    ) of Gal308 for ONPG and lactose are determined. The k     _cat    and K     _m    values are 464.7±7.8 s     ^-1    and 2.7±0.3 mM for ONPG, and 264.2±2.1 s     ^-1    and 7.1±0.8 mM for lactose, respectively. The k     _cat    /K     _m    value of the enzyme for ONPG (172.1 s     ^-1    mM     ^-1    ) is 4.6-fold higher than that for lactose (37.2 s     ^-1    mM     ^-1    ), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose.

用途 

用于测定β-半乳糖苷酶活性

用途 

生化研究

用途 

用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。

安全信息

MSDS信息

2-硝基苯-beta-D-半乳糖苷 价格(试剂级)

2-硝基苯-beta-D-半乳糖苷 上下游产品信息