吡啰红Y CAS#: 92-32-0

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吡啰红Y

生物活性体外研究 用途与合成方法 MSDS 吡啰红Y价格(试剂级) 上下游产品信息

中文名称	吡啰红Y
中文同义词	派洛宁YPYRONI;PYRONING(Y) 吡啰红G 派洛宁G (Y);派洛宁Y, 高纯生物染料;派洛宁Y92-32-0;派洛宁Y(IND GRADE);吡罗红Y;派洛宁G;3,6-双(二甲基氨基)吨氯化物
英文名称	PYRONIN Y
英文同义词	Pyronin Y,Pyronin G, PyroninJ, PyroninY;Pyronin G, C.I. 45005, for microscopy;Pyronin G (Pyronin Y);Pyronin y(G);Pyronin  G,  PyroninJ;Pyronin  G,  PyroninJ,  PyroninY;Pyronin Y (CI x  45005);Pyronin Y,pure,high purity biological stain
CAS号	92-32-0
分子式	C17H19ClN2O
分子量	302.8
EINECS号	202-147-8
相关类别	血液学和组织学;色素类;色素;生物化学;染色剂和染料;生化试剂-色素类;化学试剂;生化试剂;通用生化试剂-生物染色剂与化学指示剂;Xanthene
Mol文件	92-32-0.mol
结构式

吡啰红Y 性质

吡啰红Y 用途与合成方法

生物活性

Pyronin Y (Pyronine G) 是一种可插入 RNA 的阳离子染料，可以靶向包括 RNA，DNA 和细胞器的细胞结构。Pyronin Y 与双链核酸(尤其是 RNA) 形成荧光复合物，可以对细胞 RNA 进行半定量分析。Pyronin Y 可用于鉴定活细胞中核糖核蛋白复合物的特定 RNA 亚种。

体外研究

Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin.  
 A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting.  
 In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells.  
 Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent.  
 Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA).  

用途 

一种插入RNA的阳离子染料，在电泳过程中呈红色带。

安全信息

MSDS信息

吡啰红Y 价格(试剂级)

吡啰红Y 上下游产品信息