BETA淀粉样蛋白片段1-40 CAS#: 131438-79-4

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BETA淀粉样蛋白片段1-40

生物活性体外研究体内研究

中文名称	BETA淀粉样蛋白片段1-40
中文同义词	BETA淀粉样蛋白片段1-40;Β 淀粉样蛋白片段1-40;Β 淀粉样蛋白片段1-40, ≥95%(HPLC),POWDER;Β-AMYLOID 1-40;淀粉样Β-蛋白(人,1-40);AΒ40;Β淀粉样肽1-40;Β-淀粉样蛋白(1-40),人
英文名称	AMYLOID BETA-PROTEIN (HUMAN, 1-40) TRIFLUOROACETATE
英文同义词	AMYLOID B-PROTEIN FRAGMENT 1-40;Amyloidb-Peptide(1-40)(human);H-Asp-Ala-Glu-Phe-Gly-His-Asp-Ser-Gly-Phe-Glu-Val-Arg-His-Asp-Ser-Gly-Phe-Glu-Val-Arg-His-Gln-Lys-Leu-Val-Gly-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-OH;Amyloid beta Protein fragment 1-40 HCl Salt;amyloid β protein fragment 1-40;aβ40;SS-AMYLOID (1-40), RAT;M.W. 4329.86   C194H295N53O58S
CAS号	131438-79-4
分子式	C194H295N53O58S
分子量	4329.8034
EINECS号
相关类别	多肽;多肽与蛋白;活性多肽;试剂;细胞生物学;细胞信号和神经生物学;多肽，氨基酸;分子生物试剂-多肽与蛋白;药物多肽;其他生化试剂;药物多肽;proteins;Peptide;Amyloid beta-peptide and related;Neuroscience
Mol文件	131438-79-4.mol
结构式

BETA淀粉样蛋白片段1-40 性质

BETA淀粉样蛋白片段1-40 用途与合成方法

生物活性

β-Amyloid (1-40) 是阿尔茨海默病患者脑斑块中发现的主要蛋白质。

体外研究

β-Amyloid (1-40) and (1-42) are major components of senile plaque amyloids, are physiological peptides present in the brain, cerebrospinal fluid (CSF) and plasma. The levels of CSF β-Amyloid (1-40) and (1-42) show a U-shaped natural course in normal aging.Chronic infusion of beta-amyloid (1-40) for 14 days into the rat cerebroventricle decreased the activity of soluble protein kinase C (PKC) in the hippocampus. Subcellular translocation of PKC to membrane fraction in hippocampal slices of rats treated with beta-amyloid (1-40) is completely abolished under acute stimulation with 0.5 microM phorbol-dibutyrate (PDBu).The further aggregation of β-Amyloid (1-40)1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80°C.3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.4. Next, the solution was age 48h at 4-8°C. The sample was then centrifuged at 14000g for 10 min at 4-8°C; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.Methods vary depends on the downstream applications.

体内研究

Chronic infusion of β-Amyloid (1-40) into rat cerebroventricle leads to deficit in spatial and non-spatial memory formation. Chronic treatment of β-Amyloid (1-40) does not change lever-pressing performance significantly, but performance declined significantly 30 days after termination of the chronic daily regimen. The soluble unaggregated form of β-Amyloid (1-40), injected into the dorsal hippocampus, does not appear to have behavioral effects on performance or short-term working memory in rats, but multiple repeat injections produced performance decrements several weeks later. Repeated injection of β-Amyloid (1-40) through indwelling cannulae shows promise for development of an animal model of Alzheimer's disease.

参考质量标准

外观:白色粉末
纯度(HPLC) ≥95.0%
醋酸根含量5.0%～12.0%
水分含量≤8.0%
肽含量≥80.0%

安全信息

MSDS信息

BETA淀粉样蛋白片段1-40 上下游产品信息