FLAG肽 CAS#: 98849-88-8

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FLAG肽

生物活性体外研究 用途与合成方法 MSDS FLAG肽价格(试剂级) 上下游产品信息

中文名称	FLAG肽
中文同义词	DDDDK肽;L-LYSINE, L-Α-ASPARTYL-L-TYROSYL-L-LYSYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-;FLAG® PEPTIDE;FLAG PEPTIDE 试剂;化合物FLAG PEPTIDE;FLAG TAG多肽;FLAG肽;FLAG多肽
英文名称	FLAG peptide
英文同义词	FLAG tag Peptide;FLAG PEPTIDE|DYKDDDDK|ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;FLAG Tag Peptide，FLAG epitope;FLAG®FLAG PEPTIDE, >98%;FLAG peptide,DYKDDDDK,Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, >98%;(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-amino-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]hexanoic acid;Inhibitor,FLAG peptide,inhibit
CAS号	98849-88-8
分子式	C41H60N10O20
分子量	1012.97
EINECS号
相关类别	植物生化提取物;生物功能肽;多肽;目录多肽;peptides;EpitopeTags
Mol文件	98849-88-8.mol
结构式

FLAG肽 性质

FLAG肽 用途与合成方法

生物活性

FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。

体外研究

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca     ²⁺    . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca     ²⁺    and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.

多肽

FLAG Peptide（Flag-tag Peptide）是编码8个氨基酸的亲水性多肽（DYKDDDDK），含有肠激酶酶切位点，可用于免疫亲和层析。Flag多肽可在非变性条件下洗脱蛋白，有多种抗体可识别Flag多肽，如M1、M2和M5。

参考质量标准

外观:白色粉末
纯度(HPLC) ≥98.0%
醋酸根含量≤12.0%
水分含量≤8.0%
肽含量≥80.0%
内毒素≤50EU/mg
氨基酸组成分析≤±10%

安全信息

MSDS信息

FLAG肽 价格(试剂级)

FLAG肽 上下游产品信息