双链聚肌胞 CAS#: 42424-50-0

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双链聚肌胞

生物活性靶点体外研究体内研究 用途与合成方法 MSDS 双链聚肌胞价格(试剂级) 上下游产品信息

中文名称	双链聚肌胞
中文同义词	聚肌苷酸-聚胞苷酸钠盐;双链聚肌胞;6.双链聚肌胞(POLYI:C .);卡那霉素-聚肌胞钠盐复合物;双链聚肌胞钠盐;双链聚肌胞溶液;POLYI:C双链聚肌胞钠盐;聚肌苷 - 聚胞苷酸钠盐
英文名称	Polyinosinic-polycytidylic acid sodium salt
英文同义词	POLYINOSINIC ACID + POLYCYTIDYLIC ACID, SODIUM SALT;Polymyocyte sodium salt;POLYINOSINIC ACID POLYCYTIDYLIC ACID, SODIUM SALT;POLYINOSINIC-POLYCYTIDYLIC ACID SODIUM SALT;POLY I POLY C, NA;POLY[I] POLY[C] SODIUM SALT;Oligochitosan-kanamycin-polyI-polyC  sodium salt compound solution;Polyinosinic acid - polycytidylic acid sodium salt, double-stranded
CAS号	42424-50-0
分子式	C19H27N7O16P2
分子量	671.41
EINECS号	1308068-626-2
相关类别	医药中间体;中间体;兽用鉰料添加剂和婴幼儿奶粉添加;医药兽药;原料药;生化试剂-聚合物类;医药原料;化学试剂;Biochemicals and Reagents;Biomaterials;Cell Culture;Materials Science;Nucleosides;Nucleotides;Oligonucleotide Polymers and Copolymers;Oligonucleotides;Other adjuvants;Synthetic Oligonucleotides and Related Reagents;Vaccine Adjuvants;Vaccine Production
Mol文件	42424-50-0.mol
结构式

双链聚肌胞 性质

双链聚肌胞 用途与合成方法

生物活性

Polyinosinic-polycytidylic acid sodium (Poly(I:C) sodium) 是双链 RNA 的合成类似物，是一种 TLR3 和视黄酸诱导型基因 I 受体 (RIG-I 和 MDA5) 的激动剂。Polyinosinic-polycytidylic acid sodium 可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡。

靶点

TLR3, RIG-I, MDA5, apoptosis

体外研究

Polyinosinic-polycytidylic acid (20 ng/mL; 24 hours; WM793, WM278, WM239A, WM9 and 1205Lu cells) treatment strongly reduces viability from 100% in controls to 20%–50% within 24 hours.  
 Polyinosinic-polycytidylic acid (200 ng/mL; 24 hours; 1205Lu cells) treatment induces apoptosis in 1205Lu cells.  
 Polyinosinic-polycytidylic acid (3 ng/mL; 24 hours; 1205Lu cells) treatment induces IFN-β expression in melanoma cells. Silencing of RIG-I and MDA-5 confirmed that induction of IFN-β by Polyinosinic-polycytidylic acid required RIG-I and MDA-5, respectively, and that required IPS-1.  
 Polyinosinic-polycytidylic acid (5 ng/mL; 24 hours; 1205Lu cells) treatment reveals active subunits of caspase-9 and caspase-8 in melanoma cells.  
 Polyinosinic-polycytidylic acid sodium is prepared for injection by resuspending in sterile saline, heating to 50 °C at a concentration of 2 mg/ml to ensure complete solubilisation and then allowing to cool naturally to room temperature to ensure proper annealing of double-stranded RNA. Poly I:C is stored at -20 °C until use.  

Cell Viability Assay

Apoptosis Analysis

RT-PCR

Western Blot Analysis

体内研究

Polyinosinic-polycytidylic acid treatment inhibits tumor growth in NOD/SCID immunodeficient mice injected with 1205Lu cells. The level of human DNA is 50% lower in mice treated with Polyinosinic-polycytidylic acid.

用途 

一种可作为在TLR3水平对细胞信号转导进行研究的模型RNA的双链均聚物。也是一种TRIF依赖性toll样受体-3(TLR3)配体。

安全信息

MSDS信息

双链聚肌胞 价格(试剂级)

双链聚肌胞 上下游产品信息