罗丹明 CAS#: 13558-31-1

罗丹明

吸收及优点生物活性体外研究体内研究 用途与合成方法 MSDS 罗丹明价格(试剂级) 上下游产品信息 专题

中文名称	罗丹明
中文同义词	罗丹明110, 激光级;罗丹明110(99%);罗丹明 110 氯化物;罗丹明110;罗明丹110;氯化罗丹明 110;罗丹明;罗丹明560
英文名称	RHODAMINE 110
英文同义词	R110;RHODAMINE 110;RHODAMINE 110 CHLORIDE;RHODAMINE 560;RHODAMINE 110 CHLORIDE, FOR FLUOR-ESCENC E;Rhodamine 110, laser grade, pure, 99%;2-(6-Amino-3-imino-3H-xanthen-9-yl)-benzoic acid hydrochloride;Exciton Rhodamine 560 Chloride
CAS号	13558-31-1
分子式	C20H15ClN2O3
分子量	366.8
EINECS号	236-944-7
相关类别	血液学和组织学;其它试剂;其他生化试剂;染色剂和染料;Amines;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;Q-R;Stains  and  Dyes;Stains&Dyes,  A  to
Mol文件	13558-31-1.mol
结构式

罗丹明 性质

罗丹明 用途与合成方法

吸收及优点

罗丹明类探针的优点是内酰胺环打破了氧杂蒽的π共轭，导致极低的吸收和荧光背景；在分析物的作用下，开环后可以恢复罗丹明的颜色和荧光，实现高信号/背景比分析。但是衍生物开环后通常在500 ~ 550 nm处出现最大吸收峰，在520 ~ 580 nm处发出荧光，这对于生物成像来说波长相对较短。

生物活性

Rhodamine 110 是一种灵敏和选择性的底物 (substrate)，用于测定溶液或活细胞内的蛋白酶。激发波长为 498 nm，发射波长为 521 nm。

体外研究

Rhodamine 110 accumulates in mitochondria in a cationic form, which alters the pH in this cellular compartment. Rhodamine 110 accumulates in human lymphoblastoid cells and Friend leukemia cells. No cytotoxicity to human lymphoblastoid cells is observed below 10 μM, but Rhodamine 110 causes Friend leukemia cells to die at a concentrations above 100 μM.

体内研究

Rhodamine 110 is less toxic than the parent molecule based on the intravenous LD     ₅₀    acute toxicity values of 89.5 mg/kg and 140.0 mg/kg for Rhodamine B and Rhodamine 110, respectively. Both molecules induce liver and kidney enlargement after ingestion, and male rats show more significant increases than female rats after Rhodamine 110 exposure. In addition, testis weight increased in male rats dosed with Rhodamine 110. The pharmacokinetics of Rhodamine 110 are assessed following oral administration at two dosages (3 and 10 mg/kg) and intravenous administration at one dosage (3 mg/kg). Pharmacokinetic parameters are calculated using an extravascular input and IV-bolus input, noncompartmental model analysis conducted with WinNonlin Standard Edition. The pharmacokinetic parameters of Rhodamine 110 indicates that the maximum plasma concentrations (C     _max    ) of the two oral dosages are 283.4 and 657.0 ng/mL, which are reached at 140 and 210 min, respectively. This indicates that Rhodamine 110 absorption is not rapid after ingestion, as it took over 2 h to be absorbed from the intestines into the blood. The areas under the concentration–time curves (AUCs) for the two dosages are 138.1±20.3 and 444.0±170.8 h ng/mL. The pharmacokinetic data demonstrate that the AUC is proportional to the administered oral dose of Rhodamine 110 (3 mg/kg and 10 mg/kg). Furthermore, the clearance (Cl) of the two orally administered doses is 7.94 and 8.61 mL/min/kg, respectively.

用途 

激光染料。

安全信息

MSDS信息

罗丹明 价格(试剂级)

罗丹明 上下游产品信息

上游原料

盐酸乙酸乙酯硫酸氢氧化铵苯酐氧化铝3-氨基苯酚