T Cell Enrichment Columns
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.
CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 45% and 65% of initial CD3+ cells loaded. The purity of recovered cells ranges from 81% to 88%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.
Features
Available for human, mouse, and rat cells
Purifies CD3+ T cells to 81 – 88%
Based upon gravitational flow
Simple procedure
No specialized equipment required
Kit Contents
Cell Selection Column
10X Column Buffer
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD3
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
Entrez Gene IDs:
916 (Human); 12503 (Mouse)
Alternate Names:
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
