Hard-Shell 96-well 480 PCR plates are optimized to work on the Roche LightCycler 480 real-time PCR system. Hard-Shell plates are specifically designed to withstand the stresses of heat sealing, thermal cycling, and robotic handling. The patented* two-component microplate design provides superior stability and flatness, allowing precise positioning for automation. The low-profile wells are optimized for low-volume reactions and fast PCR.
Key Features and Benefits
Polycarbonate shell — prevents plate warping when thermal cycling in the Roche LightCycler 480
Extremely uniform wells — reduce well-to-well variability in optical assays performed in real-time PCR
Black lettering — facilitates well identification
Rigid shell — ideal for heat sealing methods with instruments such as the PX1™ PCR plate sealer
Plate Dimensions
Not drawn to scale.
All dimensions in mm, unless otherwise noted.
| Microplate Dimensions | Hard-Shell® 96-Well 480 PCR Plates (HSR-99xx) |
| Length at base plane | 127.76 mm |
| Width at base plane | 85.48 mm |
| Height overall | 15.51 mm |
| Well depth | 14.41 mm |
| Well diameter at opening | 5.46 mm |
| Well diameter at bottom of conical section | 3.00 mm |
| Well volume | 200 μl |
| Well spacing | 9.00 mm |
| Well angle | 17.0° |
| Well offset | |
| Left edge to well A1 | 14.38 mm |
| Top edge to well A1 | 11.24 mm |
| Center of A1 to center of A12 | 99.00 mm |
| Center of A1 to center of H1 | 63.00 mm |
Additional Details
White-well option for increased fluorescent signal strength
Bar-coded on row A side for convenient database tracking
Footprint and well spacing match ANSI/SBS standard dimensions
Reaction volumes of 5–125 µl (200 µl maximum)
Process sampling and testing to ensure that plates are free of DNase, RNase, and human DNA
Compatible PCR Instrument
Roche LightCycler 480 real-time PCR system
* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.
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| Well/tube format | 96-well |
| Well/tube profile | Low |
| Skirt format | Engineered for optimal fit in Roche LightCycler 480 |
| Compatible PCR instrument | Roche LightCycler 480 |
| Well/tube color | White |
