生物来源
human dermis (fibroblast)
重编程方法
Sendai virus
描述
age (60-64)
mfr. no.
EBiSC
性别
female
生长模式
adherent (pluripotent)
应用
cell culture | stem cell: suitable
相关疾病
none
运输
dry ice
储存温度
−196°C
相关类别
Stem Cells
一般描述
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
细胞系来源
Depositor
Wellcome Trust Sanger Institute
细胞系描述
Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Location of primary tissue procurement: Arm
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Characterization
Analysis of Undifferentiated Cells
Marker expression: NANOG(+)POU5F1 (OCT-4)(+)SOX2(+)
PluriTest: Pluripotency score: 31.833
Novelty score: 1.289
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Genotyping
Genome-wide analysis:
Exome sequencing
联系
Additional Cell Line Info
EBiSC Access Use Agreement (EAUA)
Cell Line Information Pack (CLIP)
Note: EAUA and CLIP must be completed before order fulfillment
传代培养常规
Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
CO2 concentration: 5%
Culture medium: TeSR® E8®
Passage Number banked (pre-EBiSC): 29
法律信息
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
TeSR is a registered trademark of WiCell Research Institute, Inc.
