Species Reactivity
Human, Mouse, Rat
Specificity
Detects human NLRP10/Pynod/NALP10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1‑323), rhNALP1 (aa 1331‑1429), rhNALP2 (aa 1‑208), rhNALP3 (aa 3‑223), rhNALP3 (aa 540‑689), rhNALP5 (aa 469‑635), rhNOD1 (aa 830‑953), or rhNOD2 (aa 230‑450) is observed.
Source
Monoclonal Mouse IgG 2B Clone # 578502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NLRP10/Pynod/NALP10
Met386-Ile655
Accession # Q86W26Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.5 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human, Mouse, and Rat NLRP10/Pynod/NALP10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Catalog # MAB6606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for NLRP10/Pynod/ NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP10/Pynod/NALP10
NLRP10, also known as Pynod, NALP10, and NOD8, is a 75 kDa cytoplasmic protein that associates with the ASC component of inflammasomes. NLRP10 inhibits ASC‑dependent apoptosis, NFkB activation, and Caspase-1-dependent processing of IL-1 beta. Human NLRP10 contains one Pyrin domain (aa 1-96) and one NACHT domain (aa 167-484) but no leucine-rich repeats. Within aa 386-655, human NLRP10 shares approximately 50% aa sequence identity with mouse and rat NLRP10.
Long Name:
NLR Family, Pyrin Domain Containing 10
Entrez Gene IDs:
338322 (Human); 244202 (Mouse); 293426 (Rat)
Alternate Names:
CLR11.1; NACHT, leucine rich repeat and PYD containing 10; NALP10; NALP10Pynod; NLR family, pyrin domain containing 10; NLRP10; NOD8; NOD8Nucleotide-binding oligomerization domain protein 8; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 10; PAN5; Pynod; PYNODNACHT, LRR and PYD domains-containing protein 10
