Species Reactivity
Human
Specificity
Detects HEK293 cells transfected with human CHRM3 in Cell-based ELISA
Source
Monoclonal Mouse IgG 2B Clone # 580011
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CHRM3
Accession # P20309Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreas
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CHRM3
Muscarinic acetylcholine receptor M3 (CHRM3) is a 65 kDa 7-transmembrane glycoprotein that is predominantly expressed in the central nervous system, smooth muscle, and heart. CHRM3 mediates signal transduction in the autonomic nervous system in response to muscarinic agonists. Human CHRM3 shares 92% aa sequence identity with mouse and rat CHRM3.
Long Name:
Cholinergic Receptor Muscarinic 3
Entrez Gene IDs:
1131 (Human); 12671 (Mouse); 24260 (Rat)
Alternate Names:
cholinergic receptor, muscarinic 3; CHRM3; HM3; m3 muscarinic receptor; mAChR M3; muscarinic acetylcholine receptor M3
