Species Reactivity
Rat
Specificity
Detects rat Reg3A in direct ELISAs and Western blots. In these formats, shows 100% cross-reactivity with recombinant mouse (rm) Reg3A and no cross-reactivity with rmReg1, recombinant human (rh) Reg1B, recombinant rat Reg2, or rhReg4.
Source
Monoclonal Mouse IgG 2A Clone # 250802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat Reg3A
Glu26-Gln174
Accession # NP_742074Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
Recombinant Rat Reg3A
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg3A
Reg3A, also named lithostathine 3 and pancreatitis-associatedprotein 2, belongs to the Regenerating gene family of C-type lectins. Reg3A is overexpressed during the acute phase of pancreatitis.
Long Name:
Regenerating Islet-derived IIIa
Entrez Gene IDs:
5068 (Human); 19694 (Mouse); 171162 (Rat)
Alternate Names:
hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; Reg3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III
