Species Reactivity
Human
Specificity
Detects human Tryptophan Hydroxylase 1/TPH‑1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tryptophan Hydroxylase 1/TPH‑1
Met102-Pro403
Accession # P17752Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human Tryptophan Hydroxylase 1/TPH‑1 by Western Blot. Western blot shows lysates of AGS human gastric adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tryptophan Hydroxylase 1/TPH‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # ). A specific band was detected for Tryptophan Hydroxylase 1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunohistochemistry | Tryptophan Hydroxylase 1/TPH‑1 in Human Brain. Tryptophan Hydroxylase 1/TPH‑1 was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human Tryptophan Hydroxylase 1/TPH‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for . |
Immunohistochemistry | Tryptophan Hydroxylase 1/TPH‑1 in Rat Brain. Tryptophan Hydroxylase 1/TPH‑1 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Human Tryptophan Hydroxylase 1/TPH‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5276) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to neurons. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tryptophan Hydroxylase 1/TPH-1
Tryptophan Hydroxylase 1 (TPH-1) is a 50‑60 kDa intracellular enzyme that belongs to the biopterin-dependent, aromatic amino acid hydroxylase family. It is expressed in gut enterochromaffin cells and pineal gland. TPH-1 catalyzes the hydroxylation of L-Trp, which is the rate-limiting step in serotonin biosynthesis. Human TPH-1 is 444 amino acids (aa) in length. It contains an N-terminal regulatory domain (aa 1‑98), a catalytic region (aa 99‑424), and a C‑terminal Leu-zipper tetramerization domain (aa 425‑444). TPH-1 is phosphorylated on Ser58, and subsequently binds to 14-3-3 proteins, resulting in activation. It functions as a noncovalent Fe-binding 230 kDa homotetramer. There are three potential splice variants. One shows a 29 aa substitution for aa 438‑444, a second shows a 50 aa substitution for aa 157‑444, and a third shows a 68 aa substitution for aa 1‑21. Over aa 102‑403, Human TPH-1 shares 95% aa identical with mouse TPH-1.
Entrez Gene IDs:
7166 (Human); 21990 (Mouse); 24848 (Rat)
Alternate Names:
EC 1.14.16; EC 1.14.16.4; indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; TPH-1; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); Tryptophan Hydroxylase 1
