Species Reactivity
Mouse
Specificity
Detects mouse SCD-1 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG 2A Clone # 428208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse SCD-1
Met1-Ser355
Accession # P13516Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
Recombinant Mouse SCD‑1
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCD-1
Stearoyl-CoA Desaturase 1 (SCD-1) is a four-transmembrane endoplasmic reticulum protein that is a rate-limiting enzyme in the formation of triglycerides, cholesteryl esters and phospholipids from saturated fatty acids. Its expression in adipose and liver is enhanced by insulin and certain dietary sugars and fats, and down‑regulated by TNF-alpha. SCD-1 is one of four iron-containing murine stearoyl-CoA desaturases that share 79‑88% aa identity. The 355 aa, predicted 41 kDa mouse SCD-1 shares 92% and 84% aa identity with rat and human SCD-1, respectively.
Long Name:
Stearoyl CoA-desaturase 1, Isoform of the Delta9 Desaturase
Entrez Gene IDs:
6319 (Human); 20249 (Mouse)
Alternate Names:
FADS5; MSTP008; SCD1; SCD-1; SCDOS; Stearoyl-CoA desaturase
