Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10 4 cells/well are added to rhICAM-2/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >70% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.Source
Mouse myeloma cell line, NS0-derived
Human ICAM-2
(Lys25 - Gln223)
Accession # CAA33630IEGRMD Human IgG1
(Pro100 - Lys330)6-His tag N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisLys25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa (monomer)
SDS-PAGE
86 kDa, reducing conditions
803-I2 |
| |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol. | ||
Reconstitution Reconstitute at 0.4 mg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICAM-2/CD102
Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.
References:
Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name:
Intercellular Adhesion Molecule 2
Entrez Gene IDs:
3384 (Human); 15896 (Mouse)
Alternate Names:
CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2
