Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Ube2E3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ube2E3 concentration of 0.1-1 μM.
Source
E. coli-derived Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
25 kDa
E2-678 |
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Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1mM TCEP | ||
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: UBE2E3
Ubiquitin-conjugating Enzyme E2E 3 (UBE2E3), also known as Ubiquitin-conjugating Enzyme H9 (UbcH9), is a member of the Ubiquitin-conjugating (E2) enzyme family (1). UBE2E3 has a predicted molecular weight of 23 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2E3 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (2). This enzyme appears to be localized in the nucleus of interphase cells and cell-wide in mitotic cells (3). UBE2E3 may be regulated at the post-transcriptional level by miR-143 (4). The auto-ubiquitination of the ARA54, RNF8, and bacterially-encoded NleG Ubiquitin ligases (E3s) is mediated by UBE2E3 (5,6). UBE2E3 also functions with NEDD4L to regulate ENaC activity (7). Physiologically, UBE2E3 is required for the proliferation of retinal pigment epithelial cells via the negative regulation of p27/Kip1 levels (3). UBE2E3 may also be required for HIV-1 replication (8). This protein has an N-terminal His6-tag. (Accession # NP_006348.1)
References:
Ito, K. et al. (1999) Cytogenet. Cell Genet. 84:99.
Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
Plafker, K.S. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5611.
Trakooljul, N. et al. (2010) Anim. Genet. 41:357.
Ito, K. et al. (2001) Eur. J. Biochem. 268:2725.
Wu, B. et al. (2010) PLoS Pathog. 6:e1000960.
Debonneville, C. et al. (2004) Mol. Cell. Biol. 24:2397.
Dziuba, N. et al. (2012) AIDS Res. Hum. Retroviruses 28:1329.
Entrez Gene IDs:
10477 (Human); 22193 (Mouse); 295686 (Rat)
Alternate Names:
E2-23K; EC 6.3.2.19; UBCE4; UbcH9; UBCH9ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); UbcM2; UBE2E3; Ubiquitin carrier protein E3; ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E3
