Format
96-well strip plate
Assay Length
< 1 hour
Sample Type & Volume Required Per Well
Urine (10 uL)
Sensitivity
0.07 mg/dL
Assay Range
0.3 - 20 mg/dL (Urine)
Specificity
Measures Creatinine levels in urine
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with other substances.
Control Available
<div class="add-to-cart" data-type="anc" data-catbase="QC69" data-return="creatinine-parameter-assay-kit_kge005" data-produrl="parameter-immunoassay-control-set-685-for-creatinine_qc69" data-title="Parameter Immunoassay Control Set 685 for Creatinine" data-catnums="QC69">
QC69, Parameter Immunoassay Control Set 685 for Creatinine -
Product Summary
The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.67 | 5.28 | 9.75 | 1.55 | 4.92 | 9.25 |
| Standard Deviation | 0.053 | 0.168 | 0.339 | 0.085 | 0.259 | 0.366 |
| CV% | 3.2 | 3.2 | 3.5 | 5.5 | 5.3 | 4 |
Recovery
The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Urine (n=4) | 96 | 92-97 |
Linearity
To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.
Preparation and Storage
Storage
Storage conditions vary by component. See product insert for storage instructions.
Background: Creatinine
Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.
Alternate Names
Creatinine;
