Species Reactivity
Human, Mouse
Specificity
Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.
Source
Monoclonal Rat IgG 2A Clone # 261034
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MSK2
Met1-Ser772
Accession # O75676Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human/Mouse MSK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse MSK2 Monoclonal Antibody (Catalog # MAB2310) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK2 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MSK2
Mitogen- and stress-activated protein kinase 2 (MSK2), also known as ribosomal protein S6 kinase B (RSKB) and RPS6KA4, belongs to the family of AGC kinases. Both MSK2 and the related MSK1 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.
Long Name:
Mitogen- and Stress-activated Protein Kinase 2
Entrez Gene IDs:
8986 (Human); 56613 (Mouse)
Alternate Names:
MSK2; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSKB
