Species Reactivity
Mouse
Specificity
Detects mouse EOMES in direct ELISAs.
Source
Monoclonal Rabbit IgG Clone # 1219A
Immunogen
E. coli-derived recombinant mouse EOMES
Met1-Ser126
Accession # O54839Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
0.25-1 µg/10 6 cells
Mouse splenocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: EOMES
EOMES (Eomesodermin), also TBR2, is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.
Long Name:
Eomesodermin Homolog
Entrez Gene IDs:
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names:
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2
