Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human ITCH/AIP4 is a HECT domain Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ITCH/AIP4 concentration of 0.1-0.5 μM.
Source
E. coli-derived
Accession #
Predicted Molecular Mass
99 kDa
E3-260 |
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Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 2 mM TCEP | ||
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ITCH/AIP4
The mammalian Itchy homolog, or ITCH, (also known as Atrophin-1-interacting protein 4 or AIP4) is a HECT domain class ubiquitin E3 ligase. ITCH/AIP4 ubiquitinates the phosphorylated form of Dishevelled protein and promotes its degradation via the Ubiquitin Proteasome System, thereby inhibiting canonical Wnt signaling. The absence of ITCH/AIP4 has been shown to cause severe autoimmune disease in mice. Recent studies have identified multisystem autoimmune disease and morphologic and developmental abnormalities in human patients with ITCH/AIP4 deficiency, thus underscoring the importance of ITCH/AIP4 in many cellular processes. This ligase may catalyze K29-, K48-, and/or K63-linked polyubiquitin chain formation on a variety of reported targets. This untagged, ITCH/AIP4 isoform 2 recombinant protein demonstrates strong autoubiquitination activity in vitro.
References:
Wei W., et al. (2012) Mol. Cell Biol. 32: 3903-12
Lohr N.J., et al. (2010) Am. J. Hum. Genet. 86: 447-453
Yang C., et al. (2006) Mol. Cell 21: 135-141
Long Name:
Itchy E3 Ubiquitin Protein Ligase
Entrez Gene IDs:
83737 (Human); 16396 (Mouse); 311567 (Rat)
Alternate Names:
AIF4; AIP4; AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; EC 6.3.2; EC 6.3.2.-; ITCH; itchy (mouse homolog) E3 ubiquitin protein ligase; itchy E3 ubiquitin protein ligase homolog (mouse); itchy homolog E3 ubiquitin protein ligase; NAPP1; NAPP1E3 ubiquitin-protein ligase Itchy homolog; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH
