Species Reactivity
Human
Specificity
Detects human CD1e in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1d, or recombinant rat beta 2-Microglobulin is observed.
Source
Monoclonal Mouse IgG1 Clone # 704407
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD1e
Met1-Tyr304
Accession # P15812Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 350
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
0.25-1 µg/10 6 cells
Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CD1e
CD1e is an approximately 40 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. CD1e associates with beta 2-microglobulin in endosomes and the Golgi where it facilitates the processing of glycolipids for their presentation by other CD1 family proteins. CD1e is expressed by most nonhuman mammals but not by mice or rats. It contains one Ig-like domain in its extracellular region. Alternate splicing of human CD1e generates multiple isoforms with various deletions and substitutions in the extracellular and/or cytoplasmic domains.
Entrez Gene IDs:
913 (Human)
Alternate Names:
CD1-alpha-3; CD1e molecule; CD1e; CDIEL; e polypeptide; FLJ17609; membrane-associated; R2G1; thymocyte antigen CD1E
