Species Reactivity
Human
Specificity
Detects human UVRAG in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 953035
Immunogen
Human UVRAG synthetic peptide corresponding to sequences between amino acids 260-360
Accession # Q9P2Y5Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
0.25-1 µg/10 6 cells
Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: UVRAG
UV radiation resistance-associated gene protein, or UVRAG, is a multi-function protein of 699 amino acids involved in the regulation of cellular pathways implicated in membrane trafficking. UVRAG activates the Beclin-1-PI3KC3 complex, a critical element in the autophagy signaling pathway. Binding studies show that UVRAG and Beclin-1 directly interact via their coiled-coil domains and that Beclin-1 functions as a platform for the formation of the Bcl-2-UVRAG-PI3KC3 complex. Chromosomal aberrations involving the UVRAG gene are associated with left-right axis malformation, and mutations in the gene have been associated with colon cancer.
Long Name:
UV Radiation Resistance Associated
Entrez Gene IDs:
7405 (Human); 78610 (Mouse); 308846 (Rat)
Alternate Names:
beclin 1 binding protein; DHTX; disrupted in heterotaxy; p63; UV radiation resistance associated gene; UV radiation resistance-associated gene protein; UVRAG; VPS38
