Species Reactivity
Human
Specificity
Detects human TIMP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) TIMP‑1 or rhTIMP‑2.
Source
Monoclonal Mouse IgG2b Clone # 63515
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-1
Cys24-Ala207
Accession # P01033Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
0.25-1 µg/10 6 cells
Human T cells fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIMP-1
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Long Name:
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs:
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names:
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1
