Species Reactivity
Mouse
Specificity
Detects mouse TCR gamma /δ in flow cytometry.
Source
Monoclonal Hamster IgG 2 kappa Clone # GL-3/5E11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse intraepithelial lymphocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Biotin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Hamster Anti-Mouse TCR gamma /δ Biotinylated Monoclonal Antibody (Catalog # FAB7297B) or (B) Hamster IgG Biotinylated Control (Catalog # ) followed by Streptavidin-Phycoerythrin (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: TCR gamma/delta
The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).
References:
Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.
Entrez Gene IDs:
915 (Human); 12500 (Mouse); 25710 (Rat)
Alternate Names:
CD3d; delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCR gamma/delta; TCRG; TiT3; TRG
