Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Type & Volume Required
Cell lysates (100 µL)
Range
312.00 - 20,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot
Kit Content
Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP
Other Reagents Required
PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2HPO 4, 1.5 mM KH 2O 4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # ), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H 2O 2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )
Stop Solution: 2 N H 2SO 4 (Catalog # )
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # ), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ROR1
ROR1 and ROR2 are receptor tyrosine kinases characterized by an extracellular immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, a kringle domain, and an intracellular kinase domain. RORs have been implicated in cartilage and growth plate development, and neurite outgrowth.
Entrez Gene IDs:
4919 (Human);
Long Name:
Receptor Tyrosine Kinase-like Orphan Receptor 1
Aliases:
EC 2.7.10.1; MGC99659; neurotrophic tyrosine kinase receptor-related 1; Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1; NTRKR1dJ537F10.1; receptor tyrosine kinase-like orphan receptor 1; ROR1; tyrosine-protein kinase transmembrane receptor ROR1
