Species Reactivity
Human, Porcine, Equine
Specificity
Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 3, beta 4, beta 5, beta 8, recombinant mouse Integrin beta 1, beta 2, beta 6, or beta 7 is observed.
Source
Monoclonal Mouse IgG2b Clone # 419127
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1/CD29
Gln21-Asp728 (predicted)
Accession # P05556Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
Human peripheral blood mononuclear cells (PBMCs), porcine mesenchymal stem cells, and equine peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as VLA-beta chain or CD29, associates with at least ten different Integrin alpha subunits. Within aa 21-728, human Integrin beta 1 subunit is 94% aa identical to pig and sheep Integrin beta 1 subunits.
Entrez Gene IDs:
3688 (Human); 16412 (Mouse)
Alternate Names:
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
