Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SSEA-1.
Source
Monoclonal Mouse IgM Clone # MC-480
Immunogen
F9 mouse teratocarcinoma stem cells
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
D3 mouse embryonic stem cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: SSEA-1
Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).
References:
Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
Fox, N. et al. (1983) Cancer Res. 43:669.
Long Name:
Stage-specific Embryonic Antigen-1
Alternate Names:
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1
