Species Reactivity
Human
Specificity
Detects human CCRL2/CRAM‑A/B. Stains human HCR-transfected cells but not irrelevant transfectants. It detects both the CRAM-A and CRAM-B isoforms.
Source
Monoclonal Mouse IgG2b Clone # 152211
Immunogen
NS0 mouse myeloma cell line transfected with human CCRL2/CRAM‑A/B
Met1-Val356 (Val180Met)
Accession # NP_001124382Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
Human CCRL2/CRAM-A transfected NS0 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCRL2/CRAM-A/B
HCR, also known as CRAM-A, CRAM-B, CKRX and CCRL2, is a seven-transmembrane G-protein linked receptor that shares homology with other human chemokine receptors. Two isoforms were reported and designated CRAM-A and CRAM-B (1) that differ at their N-termini by the inclusion of an additional 12 amino acids on CRAM-A. HCR/CRAM is expressed at varying levels on a variety of peripheral blood cells including monocytes, neutrophils and T cells (2, 3).
References:
Fan, P. et al. (1998) Biochem. Biophys. Res. Commun. 243:264.
Migeotte, I. et al. (2002) Eur. J. Immunol. 32:494.
Long Name:
CC Chemokine Receptor-like 2
Entrez Gene IDs:
9034 (Human)
Alternate Names:
ACKR5; C-C chemokine receptor-like 2; CCR11; CCR6; CCRL2; CCRL2/CRAM-A/B; chemokine (C-C motif) receptor-like 2; Chemokine receptor CCR11; Chemokine receptor X; CKRX; CKRXMGC116710; CRAM; CRAMA; CRAM-A; CRAMB; CRAM-B; FLJ55815; HCR; HCRMGC34104; Putative MCP-1 chemokine receptor
