Species Reactivity
Human
Specificity
Detects human TRA‑1‑85 antigen in Western blots.
Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25-1 µg/10 6 cells
Human peripheral blood lymphocytes and monocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
References:
Williams, B.P. et al. (1988) Immunogenetics. 27:322.
Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.
Entrez Gene IDs:
682 (Human)
Alternate Names:
OKa Blood Group Antigen; TRA185; TRA-1-85
