Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
Monoclonal Mouse IgG 2A Clone # 303123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 PE‑conjugated Monoclonal Antibody (Catalog # FAB2188P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Carbonic Anhydrase IX/CA9
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.
References:
Pastorek, J. et al. (1994) Oncogene 9:2877.
Opavsky, R. et al. (1996) Genomics 33:480.
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs:
768 (Human); 230099 (Mouse)
Alternate Names:
CA9; CA-IX; CAIXcarbonic anhydrase 9; Carbonate dehydratase IX; Carbonic Anhydrase IX; carbonic anhydrase IXpMW1; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; MNRCC-associated antigen G250; P54/58N; RCC; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
