Species Reactivity
Human, Mouse
Specificity
Detects human and mouse IL-12 R beta 2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human IL-1 R beta 1 is observed.
Source
Monoclonal Mouse IgG 1 Clone # 305719
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
Cys28-Asn622
Accession # Q99665Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of IL‑12 R beta 2 in Human TH1 cells by Flow Cytometry. Human CD4+ peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 ug/mL plate-bound Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # ), 10 ng/mL Recombinant Human IL‑12 (Catalog # ), and 10 ng/mL Recombinant Human IL‑2 (Catalog # ) for 5 days to induce Th1 cell development, followed by restimulation with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin for 2 to 3 hours, were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # ) and Mouse Anti-Human/Mouse IL‑12 R beta 2 PE‑conjugated Monoclonal Antibody (Catalog # FAB1959P). Quadrant markers were set based on staining using Mouse IgG1 Phycoerythrin Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 2
Interleukin 12 (IL-12), the founding member of the IL-12 family of heterodimeric cytokines, is composed of two disulfide-linked 35 kDa and 40 kDa subunits. The 35 kDa subunit (p35) is a alpha -helical protein homologous to IL-6 and G-CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also a subunit of IL-23. IL-12 is an essential mediator of cellular-immunity that induces T cells and natural-killer cells to produce IFN-gamma. It is also required for the expansion and activation Th1 cells (1, 2).
The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 Receptor beta 1 (IL-12 R beta 1) and IL-12 Receptor beta 2 (IL‑12 R beta 2) subunits. IL-12 R beta 1 is a 100 kDa protein that is also a subunit of the IL-23 receptor complex. It binds IL-12/IL-23 p40 and is associated with Tyk2. IL‑12 R beta 2 is a 130 kDa protein that interacts with p35 and is associated with Jak2. Both receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 2 cDNA encodes a 862 amino acid (aa) protein with a putative 27 aa signal peptide that is cleaved to generate the mature protein with a 595 aa extracellular domain, a 24 aa transmembrane domain and a 216 aa cytoplasmic region. Human and mouse IL‑12 R beta 2 share 68% amino acid sequence identity. Whereas IL-12 R beta 1 expression has been detected in activated T cells, NK cells and B cells, the expression of IL‑12 R beta 2 is more restricted. Among T cells, IL-12 R beta 2 is absent on naive T cells. Activation of T cells via TCR up‑regulates IL‑12 R beta 2 expression on human Th1 but not Th2 cells (1‑4).
References:
Trinchieri, G. et al. (2003) Immunity 19:641.
Brombacher, F. et al. (2003) Trends in Immunol. 23:207.
Trinchieri, G. (2003) Nature Reviews Immunol. 3:133.
Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Long Name:
Interleukin 12 Receptor beta 2
Entrez Gene IDs:
3595 (Human); 16162 (Mouse)
Alternate Names:
IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2
