Species Reactivity
Human
Specificity
Detects human FATP1 in cell transfectants but not non-transfected parent cells in flow cytometry.
Source
Monoclonal Mouse IgG 2B Clone # 308420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FATP1
Met1-Leu646
Accession # Q6PCB7Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of FATP1 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human FATP1 PE‑conjugated Monoclonal Antibody (Catalog # IC3304P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: FATP1
FATP1 (Fatty Acid Transport Protein 1), also known as SLC27A1, is a 63-64 kDa protein that belongs to the ATP-dependent AMP-binding enzyme family. A type I transmembrane protein, FATP1 is one of six mammalian FATPs that catalyze an ATP-dependent esterification of long-chain fatty acids. FATP1 is expressed in cells with high rates of fatty acid uptake for storage of energy, principally in brown and white adipocytes, plus skeletal muscle myocytes. FATP1 likely functions as a homodimer, and is found in multiple locations such as cytoplasm, in intracellular membranes, and in the plasma membrane in response to insulin (in adipocytes). Full length FATP1 (amino acids 1-646) shares 89% amino acid sequence identity with mouse FATP1.
Long Name:
Fatty Acid Transport Protein 1
Entrez Gene IDs:
376497 (Human); 26457 (Mouse); 94172 (Rat)
Alternate Names:
ACSVL5FATP-1; EC 6.2.1; EC 6.2.1.-; FATP1; FATP1Solute carrier family 27 member 1; FATPlong-chain fatty acid transport protein 1; Fatty acid transport protein 1; FLJ00336; MGC71751; SLC27A1; solute carrier family 27 (fatty acid transporter), member 1
