Species Reactivity
Human
Specificity
Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG 2A Clone # 344222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of PAR2 in HT‑29 Human Colon Adenocarcinoma Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 PE-conjugated Monoclonal Antibody (Catalog # FAB3949P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR2
Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.
Long Name:
Protease-Activated Receptor 2
Entrez Gene IDs:
2150 (Human); 14063 (Mouse); 116677 (Rat)
Alternate Names:
CFIIRL1; coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; F2RL1; GPCR11; GPR11protease-activated receptor 2; G-protein coupled receptor 11; PAR2; PAR-2; PAR2G protein-coupled receptor-11; proteinase-activated receptor 2; proteinase-activated receptor-2; Thrombin receptor-like 1
