Human TGR5/GPBAR1 Phycoerythrin MAb (Clone 409522) 100 TESTS

• Species Reactivity

  Human

• Specificity

  Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Mouse IgG    _2B Clone # 409522

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human TGR5/GPBAR1    
  Met1-Asn330    
  Accession # Q8TDU6

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGR5/GPBAR1

TGR5, also known as GPR131, GPBAR1, M-BAR, and BG37, is a 46-48 kDa member of the GPCR1 family of molecules. It is a 330 amino acid (aa) 7-transmembrane glycoprotein that is found on a wide variety of cell types. These include monocytes, macrophages, Kupffer cells, skeletal muscle and pancreatic acinar cells, autonomic enteric and sensory DRG neurons, liver sinusoidal endothelial cells, intestinal L and EC (enterochromaffin) cells, gallbladder epithelium and cholangiocytes (bile duct epithelium). Notably, TGR5 expression exhibits polarity with respect to plasma membrane location, and it may appear either on the cell surface or in intracellualr membrane compartments such as the ER, nucleus or multivesicular bodies. Initially considered to be coupled to G alpha s with cAMP production, it is now known to also interact with G alpha i, activating the ERK signaling pathway. Bile acids generated from cholesterol in hepatocytes are a known ligand for TGR5. Aside from their role in fatty acid absorption, BA ligation of macrophage/Kupffer cell TGR5 results in an anti-inflammatory environment. Over the aa that constitute the TGR5 extracellular domain, human and mouse share 73% aa sequence identity; over the entire ORF, human and mouse share 83% aa sequence identity.

• Long Name:

  Bile Acid Receptor TGR5

• Entrez Gene IDs:

  151306 (Human); 227289 (Mouse); 338443 (Rat)

• Alternate Names:

  BG37; BG37hBG37; G protein-coupled bile acid receptor 1; GPBA; GPBAR1; GPCR19; GPR131; G-protein coupled bile acid receptor 1; G-protein coupled bile acid receptor BG37; G-protein coupled receptor GPCR19; M-BAR; M-BARhGPCR19; membrane bile acid receptor; Membrane-type receptor for bile acids; MGC40597; TGR5; TGR5GPCR