Species Reactivity
Human
Specificity
Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.
Source
Monoclonal Mouse IgG 2A Clone # 42705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA
Met1-Leu350
Accession # AAA59159Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR1/IL‑8 RA PE‑conjugated Monoclonal Antibody (Catalog # FAB330P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR1/IL-8 RA
The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.
Long Name:
Interleukin 8 Receptor A
Entrez Gene IDs:
3577 (Human); 227288 (Mouse); 54258 (Rat)
Alternate Names:
CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXCR1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL-8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A
