Species Reactivity
Human
Specificity
Detects human Integrin beta 7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 8, recombinant mouse Integrin beta 4, or beta 7 is observed.
Source
Monoclonal Mouse IgG 1 Clone # 473207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
Glu20-His723
Accession # P26010Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of Integrin beta 7 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin beta 7 PE‑conjugated Monoclonal Antibody (Catalog # FAB4669P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 7
Human Integrin beta 7, also known as Integrin beta P and M290 IEL Ag, is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20‑723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150‑389) and four cysteine‑rich EGF‑like repeats. One splice variant exists that shows a deletion of aa 501‑648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1 and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.
Entrez Gene IDs:
3695 (Human); 16421 (Mouse); 25713 (Rat)
Alternate Names:
Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7
