Species Reactivity
Human
Specificity
Detects human 5T4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5T4 is observed.
Source
Monoclonal Mouse IgG 1 Clone # 524744
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human 5T4
Ser31-Ser355
Accession # Q13641Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of 5T4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human 5T4 PE-conjugated Monoclonal Antibody (Catalog # FAB49751P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: 5T4
Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32‑355) that shows one Ser‑rich region followed by seven Leu-rich repeats (aa 90‑355). Over aa 31‑355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.
Long Name:
Oncofetal Antigen/Trophoblast Glycoprotein
Entrez Gene IDs:
7162 (Human); 21983 (Mouse); 83684 (Rat)
Alternate Names:
5T4 oncofetal trophoblast glycoprotein; 5T4 oncotrophoblast glycoprotein; 5T4; 5T4AG; 5T4-AG; 5T4M6P1,5T4 oncofetal antigen; M6P1; TPBG; trophoblast glycoprotein
