Species Reactivity
Human
Specificity
Detects human SOX17 in direct ELISAs.
Source
Monoclonal Mouse IgG 1 Clone # 614013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of SOX17 in Endoderm-differentiated BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells, endoderm differentiated with StemXVivo Endoderm Kit (Catalog # SC019), were stained with Mouse Anti-Human SOX17 PE‑conjugated Monoclonal Antibody (Catalog # IC19241P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: SOX17
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).
References:
Kanai-Azuma, M. et al. (2002) Development 129:2367.
Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
Long Name:
Transcription Factor SOX17
Entrez Gene IDs:
64321 (Human)
Alternate Names:
FLJ22252; SOX17; SRY (sex determining region Y)-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
