Species Reactivity
Human
Specificity
Detects human FSH R in direct ELISAs.
Source
Monoclonal Mouse IgG 2A Clone # 626717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FSH R
Accession # M65085Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of FSH R in OVCAR‑3 Human Cell Line by Flow Cytometry. OVCAR‑3 human ovarian carcinoma cell line was stained with Mouse Anti-Human FSH R PE‑conjugated Monoclonal Antibody (Catalog # FAB65591P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Flow Cytometry | Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human FSH R and eGFP was stained with Mouse Anti-Human FSH R PE‑conjugated Monoclonal Antibody (Catalog # FAB65591P). Quadrant markers were set based on control antibody staining (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: FSH R
Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.
Long Name:
Follicle Stimulating Hormone Receptor
Entrez Gene IDs:
2492 (Human); 14309 (Mouse)
Alternate Names:
follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1
