Species Reactivity
Human
Specificity
Detects human Slug in direct ELISAs.
Source
Monoclonal Mouse IgG 2A Clone # 666633
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Slug
Met1-His268
Accession # O43623Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Intracellular Staining by Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry | Detection of Slug in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Slug PE‑conjugated Monoclonal Antibody (Catalog # IC7408P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Slug
SLUG, also known as SNAI2, is a zinc-finger transcription factor. SLUG is a transcriptional repressor which may suppress E-cadherin transcription and be involved in an epithelial to mesenchymal transition (EMT).
Entrez Gene IDs:
6591 (Human); 20583 (Mouse)
Alternate Names:
MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; slug homolog, zinc finger protein (chicken); Slug; SLUGH; SLUGH1; SLUGzinc finger protein; SNAI2; snail 2; snail homolog 2 (Drosophila); SNAIL2; WS2D; zinc finger protein SNAI2
