Species Reactivity
Human
Specificity
Detects human ICOS in direct ELISAs.
Source
Monoclonal Mouse IgG 1 Clone # 669222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
Glu21-Phe141
Accession # Q9Y6W8Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of ICOS in Human CD3+ PBMCs by Flow Cytometry. Human CD3+ peripheral blood mononuclear cells (PBMCs) treated for 48 hours with 5 μg/mL PHA were stained with Mouse Anti-Human ICOS PE‑conjugated Monoclonal Antibody (Catalog # FAB6975P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: ICOS
Inducible Costimulator (ICOS), also known as AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule), CD278, and CRP-1 (CD28-Related Protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Over aa 21-141, human and mouse ICOS share approximately 70% aa sequence identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Long Name:
Inducible Co-stimulator
Entrez Gene IDs:
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names:
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850
