Species Reactivity
Mouse
Specificity
Detects mouse S1P 1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG 2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P 1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and GFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with either (A) Rat Anti-Mouse S1P1/EDG‑1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB7089N) or (B) Rat IgG2A Alexa Fluor 700 Isotype Control (Catalog # ). View our protocol for . |
Flow Cytometry | Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # ) and either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7089N) or (B) Rat IgG2A Alexa Fluor 700 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P1/EDG-1
S1P 1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P 1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P 1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.
Long Name:
Endothelial Differentiation Gene 1
Entrez Gene IDs:
1901 (Human); 13609 (Mouse)
Alternate Names:
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1
