Mouse CEACAM-1/CD66a Allophycocyanin MAb (Clone 723629) 100 TESTS

• Species Reactivity

  Mouse

• Specificity

  Detects mouse CEACAM-1/CD66a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CECAM-1, -3, -5, or -6 is observed. In Western blots, no cross-reactivity with rhCEACAM-1, -3, -4, -5, -6, or -7 is observed.

• Source

  Monoclonal Rat IgG    ₁ Clone # 723629

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-1/CD66a    
  Glu35-Gly428    
  Accession # P31809

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-1/CD66a

CEACAM-1 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 1), also known as BGP-1, CD66a and MHVR1, is a 110-120 kDa member of the CEACAM subfamily, CEA family of proteins. It has a wide expression pattern, being found on neutrophils, dendritic cells, endothelial cells, colonic epithelium and hepatocytes. It mediates cell adhesion, and appears to regulate insulin levels and signaling by interacting with the insulin receptor. It also demonstrates proangiogenic effects by inducing endothelial cells to proliferate and form capillary-like tubules. Finally, CEACAM-1 is a known receptor for mouse hepatitis virus. Mature mouse CEACAM-1 is a 487 amino acid (aa) type I transmembrane glycoprotein. Its contains a 394 aa extracellular region (aa 35-428) that shows one V-type (aa 35-142) and three C2-type (aa 147-411) Ig-like domains, plus a 74 aa cytoplasmic domain. Three alternate splice forms exist. One contains a four aa substitution for aa 455-521, a second shows a Gln substitution for aa 142-322, and a third possesses a combination of the first two splice patterns. CEACAM-1 forms homodimers. Over aa 35-428, mouse CEACAM-1 shares 56% and 70% aa identity with human and rat CEACAM-1, respectively.

• Long Name:

  Carcinoembryonic Antigen-related Cell Adhesion Molecule 1

• Entrez Gene IDs:

  634 (Human); 26365 (Mouse)

• Alternate Names:

  antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR