Species Reactivity
Human
Specificity
Detects human PAR1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG 2B Clone # 731115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PAR1
Arg27-Thr102, Ser375-Thr425
Accession # P25116Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of PAR1 in Human Peripheral Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin alpha 2b/CD41 APC‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human PAR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB3855P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # ). View our protocol for . |
Flow Cytometry | Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB3855P, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR1
Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.
Long Name:
Protease-Activated Receptor 1
Entrez Gene IDs:
2149 (Human); 14062 (Mouse); 25439 (Rat)
Alternate Names:
Cf2r; CF2RHTR; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR1; PAR1PAR-1protease-activated receptor 1; Protease-Activated Receptor 1; Thrombin receptor; ThrR; TRGPC; TRproteinase-activated receptor 1
