Species Reactivity
Human
Specificity
Detects human S1P 3/EDG‑3 in direct ELISAs.
Source
Monoclonal Mouse IgG 2A Clone # 776808
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human S1P 3/EDG-3
Accession # Q99500Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of S1P3/EDG‑3 in NS0 Mouse Cell Line Transfected with Human S1P3/EDG-3 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human S1P3/EDG-3 and eGFP was stained with either (A) Mouse Anti-Human S1P3/EDG‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB7524P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: S1P3/EDG-3
Sphingosine 1-Phosphate receptor-3 (S1P 3), also known as EDG-3, is a 378 amino acid (aa) seven-transmembrane receptor for the lysolipid phosphoric acid mediator, sphingosine 1-phosphate (S1P). S1P 3 interactions with S1P induce a range of responses including vasoconstriction, brachycardia, vascular endothelial and smooth muscle cell proliferation and migration, heart and liver fibrosis, and coagulation-induced inflammation. Extracellular portions of human S1P 3 show 74% aa identity with mouse and rat S1P 3.
Long Name:
Endothelial Differentiation Gene 3
Entrez Gene IDs:
1903 (Human); 13610 (Mouse); 306792 (Rat)
Alternate Names:
EDG3; EDG-3; EDG3FLJ37523; Endothelial differentiation G-protein coupled receptor 3; endothelial differentiation, sphingolipid G-protein-coupled receptor, 3; FLJ93220; G protein-coupled receptor, endothelial differentiation gene-3; LPB3; MGC71696; S1P receptor 3; S1P receptor EDG3; S1P receptor Edg-3; S1P3; S1PR3; sphingosine 1-phosphate receptor 3; Sphingosine 1-phosphate receptor Edg-3; sphingosine-1-phosphate receptor 3
