Species Reactivity
Human
Specificity
Detects human MS4A4A.
Source
Monoclonal Mouse IgG 2B Clone # 818112
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0 transfected with human MS4A4A
Accession # Q96JQ5Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Flow Cytometry HEK293 human embryonic kidney cell line transfected with human MS4A4A and eGFP was stained with either (A) Mouse Anti-Human MS4A4A PE‑conjugated Monoclonal Antibody (Catalog # FAB7797P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: MS4A4A
MS4A4A, also called CD20-like 1, is a 23 kDa (predicted) member of the MS4A family of four-transmembrane proteins that includes CD20 (MS4A1) and Fce RIb (MS4A2). MS4A4 cDNA is detected in hematopoietic cell lines, with highest expression in myeloid and B cell lines. Expressed sequence tags are detected in multiple organs and tissues. Human MS4A4A shares ~60% amino acid sequence identity with mouse and rat MS4A4A, if sequence gaps at the cytoplasmic N and C termini are disregarded. One potential isoform lacks aa 130-182, which includes the 3rd transmembrane segment and the first of two extracellular regions.
Long Name:
Membrane-spanning 4-domains, Subfamily A, Member 4A
Entrez Gene IDs:
51338 (Human); 666907 (Mouse); 361734 (Rat)
Alternate Names:
4SPAN1; CD20 antigen-like 1; CD20L1; CD20L1CD20-L1; Fc epsilon receptor beta subunit homolog; Four-span transmembrane protein 1; membrane-spanning 4-domains subfamily A member 4A; membrane-spanning 4-domains, subfamily A, member 4; membrane-spanning 4-domains, subfamily A, member 4A; MS4A4; MS4A4A; MS4A4MGC22311; MS4A7
