Mouse BTLA Alexa Fluor 488 Affinity Purified PAb 100 TESTS

• Species Reactivity

  Mouse

• Specificity

  Detects mouse BTLA in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human BTLA is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse BTLA    
  Glu30-Gly176 (Pro41Glu, Thr45Asn, Thr47Lys, Gln52His, Arg55Trp, Gln63Glu, Cys85Trp)    
  Accession # Q32MV9

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTLA

B- and T-lymphocyte Attenuator (BTLA), also known as CD272, is a 70 kDa, Ig-superfamily, type I transmembrane glycoprotein that is structurally similar to the CD28 family of T cell co-stimulatory or coinhibitory molecules (1‑3). Unlike CD28 family members, however, the BTLA extracellular Ig domain is an I-type rather than a V-type domain, and BTLA does not form homodimers (4). BTLA also differs from CD28 family members through the interaction of its Ig domain with the TNF superfamily member HVEM (herpesvirus entry mediator; TNFSF14) rather than with B7 family ligands (5). BTLA is a coinhibitory molecule expressed on T cells, B cells and, depending on the mouse strain, macrophages, dendritic and NK cells (6). Expression is low in naïve T cells and increased during antigen-specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve (6). BTLA apparently limits T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8⁺ memory T cells that are hyper-responsive to TCR crosslinking (7). The 306 amino acid (aa) BTLA contains a 29 aa signal sequence, a 154 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail that mediate inhibitory signaling (8, 9). The binding of the BTLA to HVEM does not preclude additional binding of a mammalian stimulatory HVEM ligand, either LIGHT or lymphotoxin-alpha to the complex (4). At least three alleles varying by up to ten extracellular amino acids occur in different mouse strains (6). The ECD of C57BL/6 BTLA shows 51%, 77% and 40% aa identity to that of human, rat and canine BTLA, respectively. A splice variant lacking the Ig domain, termed BTLAs, has been reported (3).

• References:

1. Murphy, K. M. et al. (2006) Nat. Rev. Immunol. 6:671.

2. Croft, M. (2005) Trends Immunol. 26:292.

3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.

4. Compaan, D. M. et al. (2005) J. Biol. Chem. 280:39553.

5. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.

6. Hurchla, M. A. et al. (2005) J. Immunol. 174:3377.

7. Krieg, C. et al. (2007) Nat. Immunol. 8:162.

8. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.

9. Chemnitz, J. M. et al. (2006) J. Immunol. 176:6603.

• Long Name:

  B- And T-Lymphocyte Attenuator

• Entrez Gene IDs:

  151888 (Human); 208154 (Mouse)

• Alternate Names:

  B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743